Regulatory

Part:BBa_K1987042

Designed by: Caroline Blassick   Group: iGEM16_UIUC_Illinois   (2016-10-14)


off -> on (mid) promoter

This promoter can be inserted upstream of any gene in order to provide timed gene expression. This promoter is inactive during the early growth phase of e. coli, but becomes more active later on, leading to an "on -> off" expression pattern.

  • Note that this promoter also includes an unidentified RBS somewhere in the sequence. This promoter+RBS combination is intended to be treated as a single regulatory unit which cannot be subdivided, which is why it has been submitted as a "basic part."

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None